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cells h1299  (Sino Biological)


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    Structured Review

    Sino Biological cells h1299
    (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an <t>anti-CD46</t> blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.
    Cells H1299, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cells h1299/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    cells h1299 - by Bioz Stars, 2026-04
    94/100 stars

    Images

    1) Product Images from "A novel Gorilla-derived oncolytic Adenovirus with natural selective replication in cancer cells"

    Article Title: A novel Gorilla-derived oncolytic Adenovirus with natural selective replication in cancer cells

    Journal: bioRxiv

    doi: 10.64898/2026.02.26.708271

    (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an anti-CD46 blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.
    Figure Legend Snippet: (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an anti-CD46 blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.

    Techniques Used: Infection, Blocking Assay, Flow Cytometry, Expressing, Virus



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    (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an <t>anti-CD46</t> blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.
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    (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an <t>anti-CD46</t> blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.
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    (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an <t>anti-CD46</t> blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.
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    (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an <t>anti-CD46</t> blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.
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    (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an <t>anti-CD46</t> blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.
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    Image Search Results


    (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an anti-CD46 blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.

    Journal: bioRxiv

    Article Title: A novel Gorilla-derived oncolytic Adenovirus with natural selective replication in cancer cells

    doi: 10.64898/2026.02.26.708271

    Figure Lengend Snippet: (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an anti-CD46 blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.

    Article Snippet: For the evaluation of CD46 and CAR cell surface levels, 2-3 x 10 5 cells (MRC5, HUVEC, A549, NCI-H1299, NCI-H1975, NCI-H727) were collected and incubated with anti-human CD46 (1:50; mouse monoclonal; #12239-MM05, Sino Biological) or with anti-human CAR (1:50; rabbit monoclonal; #10799-R271, Sino Biological) for 30 min at 4°C.

    Techniques: Infection, Blocking Assay, Flow Cytometry, Expressing, Virus

    (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an anti-CD46 blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.

    Journal: bioRxiv

    Article Title: A novel Gorilla-derived oncolytic Adenovirus with natural selective replication in cancer cells

    doi: 10.64898/2026.02.26.708271

    Figure Lengend Snippet: (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an anti-CD46 blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.

    Article Snippet: For the evaluation of CD46 and CAR cell surface levels, 2-3 x 10 5 cells (MRC5, HUVEC, A549, NCI-H1299, NCI-H1975, NCI-H727) were collected and incubated with anti-human CD46 (1:50; mouse monoclonal; #12239-MM05, Sino Biological) or with anti-human CAR (1:50; rabbit monoclonal; #10799-R271, Sino Biological) for 30 min at 4°C.

    Techniques: Infection, Blocking Assay, Flow Cytometry, Expressing, Virus